E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus-100 (enzyme included) (ProFoldin Product Code: DSA100KE)
| Description: | Antibacterial Drug Target ׀ DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA (DNA supercoiling reaction). The DNA Topoisomerase II (Gyrase) Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA in the presence of magnesium. Therefore, when the relaxed DNA is converted into its supercoiled form, the fluorescent signal increases. The change of fluorescence intensity is used to measure the supercoiling reaction of gyrases and high throughput screen of gyrase inhibitors. This product includes all the reagents for 100 assays of E. coli gyrase DNA supercoiling activity. This product includes 600 ul of 10 x Buffer T2, 405 ul of 10 x relaxed DNA, 20 ul of 1500 x H19 dye, 450 ul of 10 x ATP, 3000 ul of 10 x H19 dilution buffer and 50 ul 100 x E.coli gyrase. |
| Order #: | PFD-DSA100KE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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