S. aureus Gyrase DNA Supercoiling Assay Plus-100 (ProFoldin Product Code: DSA100KSE)
| Description: | Antibacterial Drug Target ׀ DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA. The DNA Topoisomerase II (Gyrase) Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA. Therefore, when the relaxed DNA is converted into its supercoiled form, the fluorescent signal increases. The change of fluorescence intensity is used to measure the supercoiling reaction. This product includes 500 ul of 10 x Buffer T2, 410 ul of 10 x relaxed DNA, 22 ul of 1500 x H19 dye, 450 ul of 10 x ATP, 43 ul 7.5 uM S. aureus gyrase, 820 ul of 2 M potassium glutamate and 3000 ul of 10 x H19 dilution buffer. It is for 100 assays in a 96-well plate format. |
| Order #: | PFD-DSA100KSE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
1,155.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 (0)89 3799666-6
- Fax: +49 (0)89 3799666-99
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- Online Shop: www.mobitec.com
- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
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