96-Well E. coli Topo IV DNA Decatenation Assay Kit Plus (enzyme included) (ProFoldin Product Code: EDD96KE)
| Description: | Antibacterial Drug Target ׀ Human DNA topoisomerase II and bacterial DNA topoisomerase IV (the parC-parE complex) converts the concatenated DNA into decatenated DNA (decatenation reaction). Bacterial topoisomerase I and gyrases also have DNA decatenation activity. The 96-Well Topoisomerase DNA Decatenation Assays are in a 96-well assay plate format that can be used for high-throughput tests of human topo II and bacterial topo IV inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm). This product includes 600 ul of 10 x assay buffer, 500 ul of 10 x concatenated DNA, 110 ul of 10 mM ATP, 8 ul of 1000 x E. coli topo IV, 600 ul of 0.4 M EDTA, 260 ul of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions. |
| Order #: | PFD-EDD96KE |
| Unit Size: | 96 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
1,003.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
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- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
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