Description: | Antibacterial Drug Target ׀ DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA (DNA supercoiling reaction). The Gel-Based E. coli Topoisomerase II (Gyrase) DNA Supercoiling Assay Kitis based on the principle that the supercoiled DNA and relaxed DNA are separated by agarose gel electrophoresis. Fluorescence-based DNA supercoiling assays in a 96-well pate format are also available for high throughput screening of gyrase inhibitors. This product includes 400 ul of 10 x Buffer, 105 ul of 250 ug/ml relaxed DNA, 220 ul of 10 mM ATP, 550 ul of 5 x Gel loading buffer and 25 ul of 2000 nM E.coli gyrase. |
Order #: | PFD-GDSA100KE |
Unit Size: | 100 assays |
Supplier: | ProFoldin |
Shipping: | Blue Ice (-20°C) |
Storage: | See Manual |
Subcategory: | Drug Discovery |
More information: | Go to webpage |
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