E. coli GlmU Assay Kit Plus-500 (ProFoldin Product Code: GLU500KE)
| Description: | Antibacterial Drug Target ׀ UDP-N-acetylglucosamine pyrophosphorylase (GlmU) is a bifunctional enzyme that catalyzes transfer of acetyl and uridyl groups onto glucosamine-1-P to generate UDP-GlcNAc, an essential precursor of peptidoglycans. UDP-GlcNAc is involved in the synthesis of the N-acetylglucosamine polysaccharide adhesin required for biofilm formation in bacteria. The E. coli UDP-N-acetylglucosamine pyrophosphorylase (GlmU) Assay is based on measurement of the pyrophosphate generated from the GlmU reaction. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli GlmU in drug discovery research. It may also be used for characterization of E.coli GlmU. This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x Enzyme Substrate, 0.170 ml of 100 x E. coli GlmU (500 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml), and 25 ml of Dye for phosphate detection. |
| Order #: | PFD-GLU500KE |
| Unit Size: | 500 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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