Human topoisomerase I DNA relaxation assay kit (enzyme not included) (ProFoldin Product Code: HRA020K)
| Description: | Anticancer Drug Target ׀ Human topoisomerase I relaxes supercoiled plasmid DNA to form relaxed DNA. The Human DNA topoisomerase I assay is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA. Therefore, when the supercoiled DNA is converted into its relaxed form, the fluorescent signal decreases. The change of fluorescence intensity is used to measure the relaxation reaction. This product includes 160 ul of 10 x Buffer HT1, 84 ul of 10 x supercoiled plasmid DNA, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format. Human topo I enzyme in not included and can be purchased separately. |
| Order #: | PFD-HRA020K |
| Unit Size: | 20 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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