E. coli MurA Assay Kit Plus-100 (ProFoldin Product Code: MURA100KE)
| Description: | Antibacterial Drug Target ׀ MurA or UDP-N-acetylglucosamine enolpyruvyl transferase catalyzes the first committed step in peptidoglycan biosynthesis in bacteria. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurA transfers enolpyruvate from phosphoenolpyruvate (PEP) to uridine diphospho-N-acetylglucosamine (UNAG) generating enolpyruvyl-UDPN-acetylglucosamine (EP-UNAG) and inorganic phosphate. The Bacterial MurA Assay is based on measurement of the inorganic phosphate generated from the MurA reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of bacterial MurA in drug discovery research. It may also be used for characterization of bacterial MurA. This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Assay buffer, 0.035 ml of 100 x PEP, 0.035 ml of 100 x UDP-N-acetyl glucosamine (UGN), 0.035 ml of 100 x E. coli MurA (5000 nM) and 5 ml of Dye for phosphate detection. |
| Order #: | PFD-MURA100KE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
Add to request list
- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
MoBiTec is now part of BIOZOL
All orders and requests placed via the online shop on www.mobitec.com are processed by BIOZOL Diagnostica Vertrieb GmbH.
You will receive an order confirmation by fax or e-mail. More information regarding the ordering process can be found here.
Technical Service - Product Information
Viewed