E. coli MurD Assay Kit Plus-500 (ProFoldin Product Code: MURD500KE)
| Description: | Antibacterial Drug Target ׀ MurD is a D-Glutamic acid-adding enzyme in the pathway for bacterial cell-wall peptidoglycan synthesis. It is an essential enzyme and attractive target for anti-bacterial drug discovery. MurD catalyses the addition of D-glutamic acid to UDP-MurNAc-L-Ala, generating UDP-MurNAc-dipeptide. The ligation reaction uses ATP hydrolysis as an energy source forming ADP and inorganic phosphate. The Bacterial MurD Assay is based on measurement of the inorganic phosphate generated from the MurD reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of bacterial MurD in drug discovery research. It may also be used for characterization of bacterial MurD. This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x UDP-MurNAc-L-Ala (UMA), 0.170 ml of 100 x D-Glu, 0.170 ml of 100 x ATP , 0.170 ml of 100 x E. coli MurD (2000 nM) and 25 ml of Dye for phosphate detection. |
| Order #: | PFD-MURD500KE |
| Unit Size: | 500 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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