96-Well S. pneumoniae Topo IV DNA Decatenation Assay Kit Plus (enzyme included) (ProFoldin Product Code: NDD96KE)
| Description: | Antibacterial Drug Target ׀ DNA decatenation is an essential process during DNA replication in the cells. The DNA decatenation reaction was carried out by topoisomerase IV (the parC-parE complex) in bacteria. The DNA decatenation reaction converts the concatenated DNA into decatenated DNA. The 96-Well Topoisomerase DNA Decatenation Assay is in a 96-well assay plate format that can be used for high-throughput tests of topoisomerase inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a black 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm). This product includes 600 ul of 10 x assay buffer, 500 ul of 10 x concatenated DNA, 110 ul of 10 mM ATP, 8 ul of 1000 x S. pneumoniae topo IV, 600 ul of 0.4 M EDTA, 260 ul of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions. |
| Order #: | PFD-NDD96KE |
| Unit Size: | 96 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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