NAD-dependent DNA Ligase Assay Kit (enzyme not included) (ProFoldin Product Code: NLA100K)
| Description: | Antibacterial Drug Target ׀ NAD+-dependent DNA ligases are present in bacteria, some entomopox viruses and mimi virus. Since NAD+-dependent DNA ligases are essential for bacterial growth, they are valuable targets for identifying novel antibacterial agents. The NAD+-dependent DNA Ligase Assay Kit is to measure the DNA ligase product in which the diphosphodiester bond is formed at the single stand break of a duplex DNA substrate. The ligase reaction is monitored by the fluorescence intensity at 535 nm. The assay is in 96-well plate format and can be used for screening inhibitors of DNA bacterial ligases. This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T and 220 ul of 10 x fluorescence dye for 100 assays. The kit does not include ligase that can be purchased separately (please see the information of related products). |
| Order #: | PFD-NLA100K |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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