E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit Plus (ProFoldin Product Code: PNP100KE)
| Description: | Antibacterial Drug Target ׀ Polynucleotide Polynucleotide Phosphorylase (PNPase) plays important roles in mRNA processing and degradation in various cells. RNA metabolism in bacteria involves posttranscriptional addition of poly(A) tails. PNPase has both exonuclease and polymerase activities. The E. coli PNPase assay is based on measurement of Poly(A) synthesized by E. coli PNPase using ADP. The assay can be performed in a 384-well or 96-well plate format for tests of E. coli PNPase activities and high throughput screening of inhibitors. This product includes all the reagents for 100 assays of E. coli PNPase in a 384-well plate format. The This product includes 400 ul of 10 x Buffer, 33 ul of 100 x RNA Template, 33 ul of 100 x NTPs (50 mM ATP and 50 mM GTP), 330 ul of 10 x fluorescence dye and 33 ul of 100 xE. coli PNPase (25 uM). |
| Order #: | PFD-PNP100KE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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