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Pyrophosphate Assay Reagent for RNAP (enzyme not included) (ProFoldin Product Code: PPA1000)

Pyrophosphate Assay Reagent for RNAP (enzyme not included) (ProFoldin Product Code: PPA1000)
Description: Antibacterial Drug Target ׀ RNAP Pyrophosphate Assays ׀ The bacterial RNA polymerase is responsible for biosynthesis of mRNA, tRNA and rRMA in the cells. The holoenzyme of bacterial RNA polymerases contains multiple subunits with a molecular mass of about 400 kDa. The bacterial RNA polymerase pyrophosphate assay is based on measurement of the pyrophosphate generated in the RNA polymerase reaction using a double strand DNA template. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by the phosphate detection reagent (MPA3000). The assay is performed in a 384-well plate format for high throughput screening of RNA polymerase inhibitors. This product includes 3200 ul of Buffer RP, 310 ul of 100 x NTP mix, 310 ul of 100 x DNA, 32 ul of 1000 x pyrophosphatase and 42 ml of dye for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format. The assay This product includes all reagents except the enzyme.
Order #: PFD-PPA1000
Unit Size: 1000 assays
Supplier: ProFoldin
Shipping: Blue Ice (-20°C)
Storage: See Manual
Subcategory: Drug Discovery
More information: Go to webpage
Datasheet
689.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

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