S. aureus RNA Polymerase Assay Pyrophosphate Kit Plus (ProFoldin Product Code: RPAP100KSE)
| Description: | Antibacterial Drug Target ׀ The bacterial RNA polymerase is responsible for biosynthesis of mRNA, tRNA and rRMA in the cells. The holoenzyme of bacterial RNA polymerases contains multiple subunits with a molecular mass of about 400 kDa. The bacterial RNA polymerase pyrophosphate assay is based on measurement of the pyrophosphate generated in the RNA polymerase reaction using a double strand DNA template. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by the phosphate detection reagent (MPA3000). The assay is performed in a 384-well plate format for high throughput screening of RNA polymerase inhibitors. This product includes 400 ul of Buffer RP, 33 ul of 100 x DNA, 33 ul of 100 x NTP mix, 5 ul of 1000 x pyrophosphatase, 33 ul of 100 x S. aureus RNA polymerase and 4500 ul of dye for 100 assays of S. aureus RNA polymerase reactions in a 384-well assay format. |
| Order #: | PFD-RPAP100KSE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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