96-well E. coli Gyrase DNA Cleavage Assay Kit Plus (enzyme included) (ProFoldin Product Code: T2C96KE)
| Description: | Antibacterial Drug Target ׀ Bacterial gyrase, topoisomerase IV (parC-parE ) and mammalian topoisomerase II are type II topoisomerases. These topoisomerases change DNA topology by cleavage and religation of both DNA strands. When an inhibitor such as fluoroquinolone drugs blocks the religation step of the topoisomerase reaction, the cleaved or linearized DNA forms covalent complexes with the enzyme (cleavage complex). Formation of DNA cleavage complexes causes DNA damage in the cells (topoisomerase poison). Testing formation of DNA cleavage complexes is useful for understanding the topoisomerase inhibition mechanism of novel topoisomerase inhibitors. It is also useful for toxicity evaluation regarding to human topoisomerase II. This product includes 300 ul of 10 x reaction buffer, 30 ul of 10 x DNA, 260 ul of SDS solution, 30 ul of 100 mM ATP, 260 ul of proteinase K solutions, 10 ml of TDC matrix, 6 ml of rinse buffer, 500 ul of 10 x dye, a black receiver plate, a V-bottom reaction plate, 26 ul of 100 x E. coli Gyrase and a TDC filter plate. A plate filtration device and a vacuum line are needed for the plate filtration process. |
| Order #: | PFD-T2C96KE |
| Unit Size: | 96 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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