96-Well Topoisomerase DNA Decatenation Assay Kit (enzyme not included) (ProFoldin Product Code: TDD96K)
| Description: | General Drug Target ׀ Human DNA topoisomerase II and bacterial DNA topoisomerase IV (the parC-parE complex) converts the concatenated DNA into decatenated DNA (decatenation reaction). Bacterial topoisomerase I and gyrases also have DNA decatenation activity. The 96-Well Topoisomerase DNA Decatenation Assays are in a 96-well assay plate format that can be used for high-throughput tests of human topo II and bacterial topo IV inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm). This product includes 600 ul of 10 x assay buffer, 500 ul of 10 x concatenated DNA, 110 ul of 10 mM ATP, 600 ul of 0.4 M EDTA, 260 ul of 20 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions. The assay buffer is optimized for bacterial topoisomerase IV. DNA decatenation enzyme is not included. This kit can be used for DNA decatenation assays of any bacterial topoisomerase IV (the parC-parE complex) enzymes or gyrases. |
| Order #: | PFD-TDD96K |
| Unit Size: | 96 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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