Description: | Antibacterial Drug Target ׀ Thymidylate kinase is an important enzyme in the dTTP synthesis pathway for DNA synthesis. The function of this enzyme is to catalyze the phosphorylation of dTMP using ATP to form dTDP and ADP. Thymidylate kinase is an attractive antibacterial target. The thymidylate kinase assay is based on measurement of ADP generated from the kinase reaction. The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or micro-cuvettes. This product includes 600 ul of 10 xi Buffer, 35 ul of 100 x dTMP, 35 ul of 100 x E. coli Thymidylate kinase, 35 ul of 100 x ATP, 35 ul of 100 x MUK Reagent A, 35 ul of 100 x MUK Reagent B and 300 ul of 10 x fluorescence dye for 100 assays in a 384-well assay format. |
Order #: | PFD-TMK100KE |
Unit Size: | 100 assays |
Supplier: | ProFoldin |
Shipping: | Blue Ice (-20°C) |
Storage: | See Manual |
Subcategory: | Drug Discovery |
More information: | Go to webpage |
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