mobitec-globe Innovative Tools for Molecular and Cell Biology

GFP reporter vector pGNG2

GFP reporter vector pGNG2
Usage: hover your mouse-pointer anywhere on the image above to see an enlarged portion of the image. You can also just click the image to see the original-sized version.
Description: Grow'n'Glow GFP Yeast One-Hybrid System. The Grow'n'Glow GFP One-Hybrid kit isolates genes for proteins that bind a specific DNA element of interest. In addition to finding novel DNA-binding proteins, the one-hybrid system can be used to investigate the bases and amino acids involved in specific DNA-protein interactions. Proteins can be found that bind to any short DNA element of interest. The Grow'n'Glow system offers maximal sensitivity because detection of the DNA-protein interaction occurs in vivo, where proteins are more likely to be in their native conformation. Use of the Green Fluorescent Protein (GFP) reporter eliminates the time-consuming beta-galactosidase lift assays. The particular GFP variant expressed by the GFP reporter plasmid, GFPuv, has the same excitation and emission maxima as wild-type GFP, but is 18 times brighter than the wild-type variant. Positive colonies with DNA-binding proteins glow brightly green and can be easily detected by placing the plate with the yeast colonies on a standard 300 nm UV transilluminator or under a UV lamp in a darkroom. Expression of fusion proteins by the prey plasmid is controlled by the GAL1 upstream-activation sequence. In yeast with an intact galactose regulatory system, the GAL1 activation sequence is induced by galactose and repressed by glucose. This regulation inhibits expression of library/activation domain fusions until the actual screening, which prevents any potentially toxic library proteins affecting growth of the yeast. This sort of toxic proteins would be missed during screening in a non-inducible system. Features: • Isolates genes for novel DNA-binding proteins • Maps residues and regions responsible for DNA-binding • Positive colonies fluoresce brightly green • One-step selection of potential positives under UV light • No time-consuming beta-galactosidase lift assays as essential in most conventional assays • Finds potentially toxic proteins by screening with galactose-inducible expression libraries
Order #: GNGV02
Unit Size: 5 µg
Supplier: MoBiTec GmbH
Shipping: RT
Storage: 4 °C
Subcategory: Two- and One-Hybrid Systems
Datasheet
Add to request list
    MoBiTec is now part of BIOZOL

    All orders and requests placed via the online shop on www.mobitec.com are processed by BIOZOL Diagnostica Vertrieb GmbH.
    You will receive an order confirmation by fax or e-mail. More information regarding the ordering process can be found here.

    Technical Service - Product Information
Viewed