PI3-Kinase Activity AlphaScreen Assay (Echelon Product Code: K-1300 EAKIT)
| Description: | The PI3-Kinase Activity AlphaScreen© Assay is a sensitive and robust method for the quantification of PI(3, 4, 5)P3 (PIP3), the end product of Class I PI3K reactions. This high-throughput (384-well) assay can be used for in vitro determination of PI3K activity in cancer tissues and cell lines and compound screening for drug discovery. Assay Range: 0.46 NM to 1, 000 nM Sample Type: purified enzyme, inhibitor screen Sample Volume: 2.5 µL PI3-K inhibitor Product Background When PIP3 is generated via phosphorylation of PI(4, 5)P2 (PIP2) by PI3-Kinase, the products of the enzymatic reaction compete with biotinylated I(1, 3, 4, 5)P4 for the interaction with the PIP3 detector protein. The interaction of biotinylated-I(1, 3, 4, 5)P4 and the PIP3 detector protein brings both Acceptor and Donor beads together, producing a cascade of chemical reactions and leading to the AlphaScreen© signal. In the absence of this interaction, proximity of the Donor and Acceptor beads is decreased, producing a loss of luminescent signal which is inversely related to enzyme activity. AlphaScreen© technology is based on the emission of light (520-620nm) by Acceptor beads activated by the proximity of Donor beads. Echelon has multiple formats available for Class III PI3-K. Please see the PI3-K Assay Comparison Guide below for more information. Things to consider: This product is intended for use with the Amplified Luminescent Proximity Homogenous Assay (AlphaScreen©) technology and requires the researcher to obtain donor and acceptor beads from the AlphaScreen© GST (Glutathione-S-Transferase) Detection Kit, which must be purchased separately from PerkinElmer Life Sciences. A microplate reader with AlphaScreen© capabilities is also required. |
| Order #: | ECH-K-1300-EAKIT |
| Unit Size: | EA KIT |
| Supplier: | Echelon Biosciences |
| Restrictions: | Available in all European countries, except France & Italy. |
| Shipping: | Dry Ice Or Gel Ice Depending On Destination |
| Storage: | -20 °C |
| Subcategory: | Kits & Assays |
| More information: | Go to webpage |
538.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
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