5' PtdIns(3, 4, 5)P3 Phosphatase Activity Fluorescence Polarization Assay (Echelon Product Code: K-1400 EAKIT)
| Description: | The 5' PtdIns(3, 4, 5)P3 Phosphatase Activity Fluorescence Polarization Assay determines activity through quantification of the end product, PI(3, 4)P2. Sample Type: in vitro determination of 5' phosphatase activity in cancer tissues and cell lines and compound screening for drug discovery Sample Volume: 2.5-5.0 µL enzyme / inhibitor Assay Range: 0.0625 µM to 2.0 µM Product Background: This 384 well, fluorescence polarization-based, competitive assay measures the phosphatase activity of SHIP2 which hydrolyzes the 5-phosphate from PtdInd(3, 4, 5)P(3). After the phosphatase reactions are complete, reaction products are mixed with a PI(3, 4)P(2) detector protein and the fluorescent PI(3, 4)P(2) probe. Polarization (mP) values decrease as probe binding to the PI(3, 4)P(2) detector is displaced by PI(3, 4)P(2) produced by enzymatic activity and the amount of unbound fluorescent probe in the mixture increases. |
| Order #: | ECH-K-1400-EAKIT |
| Unit Size: | EA KIT |
| Supplier: | Echelon Biosciences |
| Restrictions: | Available in all European countries, except France & Italy. |
| Shipping: | Dry Ice Or Gel Ice Depending On Destination |
| Storage: | -20 °C |
| Subcategory: | Kits & Assays |
| More information: | Go to webpage |
818.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
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- Phone: +49 (0)89 3799666-6
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