Description: | The Malachite Green Assay Kit is designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green which is quantified by reading the absorbance at 620 nm. The assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green Assay kit can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2 000 pmol of phosphate per assay point. The kit includes enough reagents for 192 samples standards and controls.The kit is offered in three sizes: K-1500: includes 2 X 96-well plates 1 vial of phosphate standard and a bottle of malachite green solution. K-1520: includes 1 X K-1500 + your choice of 2 X 100 ug lipid substrates. K-1530: includes 1 X K-1500 + your choice of 3 X 100 ug lipid substrates.Suggested available substrates: diC8 PI(3)P PI(4)P PI(5)P PI(3 4)P2 PI(3 5)P2 PI(4 5)P2 PI(3 4 5)P3The Malachite Green Solution can also be purchased separately.Phosphatase Substrate Lipid Phosphatase EnzymePtdIns(3)PMyotubularin MTMR2PtdIns(4)PPtdIns(5)PPtdIns(3 4)P2PTENPtdIns(3 5)P2OCRL1 5Ptase IV SynaptojaninPtdIns(4 5)P2MTMR2 PTPRQPtdIns(3 4 5)P3PTEN SHIP1 SHIP2 SKIP PTPRQ 5ptase II Powered by Bioz See more details on Bioz |
Order #: | ECH-K-1530-EAKIT |
Unit Size: | EA KIT |
Supplier: | Echelon Biosciences |
Restrictions: | Available in all European countries, except France & Italy. |
Shipping: | Gel Ice |
Storage: | 4-8 °C |
Subcategory: | Kits & Assays |
More information: | Go to webpage |
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