Neutral Sphingomyelinase Activity Assay Kit (Echelon Product Code: K-1800 EAKIT)
| Description: | The Neutral Sphingomyelinase Activity Assay kit is an enzyme coupled assay that measures sphingomyelinase activity in biological samples through the downstream production of choline. Assay Incubation time: 2-4 hours Assay Range: 5 mU/mL - 0.078 mU/mL Sample Type: tissue homogenates, cell lysates, serum, saliva, and urine Product Background The Neutral Sphingomyelinase Activity Assay kit can detect sphingomyelinase at concentrations as small as 78 µU/mL. The kit provides all reagents to measure the Neutral Sphingomyelinase (nSMase) activity of 40 samples ran in duplicate. In this enzyme coupled assay, N-SMase catalyzes the hydrolysis of sphingomyelin into phosphorylcholine and ceramide. Alkaline phosphatase then catalyzes phosphorylcholine to choline. Choline is them oxidized by Choline oxidase to form hydrogen peroxide. Finally, DAOS plus 4-Aminoantipyrine (4 AAP) in the presence of hydrogen peroxide and peroxidase results in the oxidative coupling of DAOS and 4 AAP to form a blue chromogen that is detected by measuring the absorbance of light at 595 nm. |
| Order #: | ECH-K-1800-EAKIT |
| Unit Size: | EA KIT |
| Supplier: | Echelon Biosciences |
| Restrictions: | Available in all European countries, except France & Italy. |
| Shipping: | Dry Ice |
| Storage: | -20 °C |
| Subcategory: | Kits & Assays |
| More information: | Go to webpage |
222.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 (0)89 3799666-6
- Fax: +49 (0)89 3799666-99
- E-mail: order@biozol.de
- Online Shop: www.mobitec.com
- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
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