SensoLyte® 520 TEV Protease Assay Kit Fluorimetric - 1 kit
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| Description: | Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal. The SensoLyte® TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity. |
| Order #: | ASP-AS-72227 |
| Unit Size: | 1 kit |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | Dry Ice |
| Storage: | - 20 °C |
| Subcategory: | Enzyme Assay Kits |
| More information: | Go to webpage |
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