SensoLyte® 520 Sortase A Activity Assay Kit Fluorimetric - 1 kit
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| Description: | Sortases are a family of membrane-anchored transpeptidases expressed by Gram-positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation. The SensoLyte® 520 Sortase A Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of protease activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. |
| Order #: | ASP-AS-72228 |
| Unit Size: | 1 kit |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | Dry Ice |
| Storage: | - 78 °C |
| Subcategory: | Enzyme Assay Kits |
| More information: | Go to webpage |
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