SensoLyte® 520 Granzyme B Activity Assay Kit, Fluorimetric - 1 kit
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| Description: | Granzymes are a family of structurally related serine proteases. They are stored within the cytotoxic granules of cytotoxic lymphocytes. Granzymes are known to induce target-cell apoptosis. They have the faculty to initiate cell death once entered the target cells (viral or tumor cells). Granzyme A and B are the most abundantly studied. Granzyme B is a serine protease mostly secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It is one of the major mediators of caspase-dependent apoptosis. Recent studies showed that Granzyme B can also be secreted by various non-cytotoxic cells and involved in degradation of extracellular matrix proteins thereby inducing inflammation and impairing wound healing. Thus, if intracellular activity of Granzyme B is mainly related to apoptosis, its extracellular activity has been linked to roles in arthritis, vascular pathologies, and other diseases. The SensoLyte® 520 Granzyme B Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of Granzyme B activity using a novel internally quenched 5-FAM/QXL®FRET substrate. Upon cleavage by Granzyme B, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The kit can be used to detect the activity of Granzyme B enzyme in biological samples and purified enzyme preparations. This assay can detect as low as 0.1 ng/mL active granzyme B. |
| Order #: | ASP-AS-72261 |
| Unit Size: | 1 kit |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | Dry Ice |
| Storage: | - 78 °C |
| Subcategory: | Enzyme Assay Kits |
| More information: | Go to webpage |
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