HS-Nuclease, recombinant Endonuclease (encoded by the same gene as Benzonase®)
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| Description: | HS-Nuclease is an ultra-pure recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Benzonase) produced in E. coli using a proprietary process. This non-specific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. HS-Nuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is free of protease activity. HS-Nuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation. Protein Type: Endonuclease. Source: E. coli. Unit Definition: One unit of HS-Nuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37oC in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 µg of salmon sperm DNA into oligonucleotides. HS-Nuclease has similar specific activity as Benzonase. Features: • Enzymatic removal of nucleic acids; cleaves all forms of DNA and RNA very efficiently; the enzyme is active on all forms of nucleic acids including single-stranded, double-stranded, linear, circular or supercoiled • Ideal for the purification of biomolecules, e.g., viral vector manufacturing • The enzyme is free of proteolytic activity • Viscosity reduction |
| Order #: | GE-NUC10700-01 |
| Unit Size: | 50000 U |
| Supplier: | MoBiTec GmbH |
| Shipping: | BI |
| Storage: | -20 °C |
| Subcategory: | Enzymes |
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