Linear polyUb Chain-binding Protein Identification / Validation Kit (UBPBio Product Code: J2520)
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| Description: | This kit is designed for two purposes: 1) identification of proteins that specially bind linear polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between linear polyUb chains and their binding proteins. The non-cleavable 6xHis-tagged linear Ub6 is resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind linear polyUb chains. After binding, 6XHis-tagged non-cleavable linear Ub6 and the binding proteins can be enriched with Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with TEV protease (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer. |
| Order #: | UBP-J2520-1 |
| Unit Size: | 1 Kit |
| Supplier: | UBPBio |
| Restrictions: | Only available in selected countries. |
| Shipping: | Dry ice |
| Storage: | -80 °C |
| Subcategory: | Kits for Ubiquitin-Proteasome Research |
| More information: | Go to webpage |
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