Nedd8 Conjugation Kit (UBPBio Product Code: J3150)
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| Description: | Nedd8 Conjugation Kit is designed for assaying the conjugation of Nedd8 to protein substrates in vitro. In a protein neddylation reaction, Nedd8 is first activated by the Nedd8 activating enzyme APPBP1/UBA3 (E1) in an ATP hydrolysis-dependent manner; activated Nedd8 is then transferred to the Nedd8 conjugating enzyme Ube2M (UbcH12) (E2) by formation of a thiolester bond with the catalytic cysteine residue of UbcH12I; Nedd8-charged Ube2M (UbcH12) can mediate protein neddylation in the presence of an appropriate Nedd8 ligase (E3). |
| Order #: | UBP-J3150-1 |
| Unit Size: | 1 Kit |
| Supplier: | UBPBio |
| Restrictions: | Only available in selected countries. |
| Shipping: | Dry ice |
| Storage: | -80 °C |
| Subcategory: | Kits for Ubiquitin-Proteasome Research |
| More information: | Go to webpage |
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