FDG [Fluorescein di-ß-D-galactopyranoside] - 5 mg
![FDG [Fluorescein di-ß-D-galactopyranoside] - 5 mg](https://www.mobitec.com/media/image/55/46/66/Labeling_600x600.jpg)
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| Description: | Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting β-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties (Ex/Em=492/520 nm) that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect β?galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In general, fluorescence-based measurements can be several orders of magnitude more sensitive than absorption-based measurements. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry. FDG is also used to detect β-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs. |
| Order #: | ASP-AS-85600 |
| Unit Size: | 5 mg |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | 4 °C |
| Storage: | - 20 °C Darkness |
| Subcategory: | Labeling & Detection |
| More information: | Go to webpage |
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