Mobicol Spin Ni-IDA
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| Description: | Ni-IDA Columns for Purification of His-tagged Proteins. Features: • Easy, fast, and cost-effective routine purification of recombinant polyhistidine-tagged proteins under native and denaturing conditions starting from diverse expression systems, e.g., E. coli, yeast, insect, and mammalian cells • Universal use: suitable for small proteins, large protein complexes, proteins with low expression rates • High binding capacity and high affinity • Improved target specificity by optimized silica-based Ni2+-IDA matrix • Imidazol-free loading and washing buffer. His-Tagged Protein Purification: Histidine-tagged protein purification uses affinity chromatography to capture recombinant proteins with 4 - 10 histidine residues. Ni-IDA columns from MoBiTec contain a silica-based resin. The form-stable silica matrix is pre-charged with Ni2+ ions and allows purification on the principle of Immobilized Metal Ion Affinity Chromatography (IMAC). Binding of proteins is based on the interaction between the polyhistidine tag of a recombinant protein and immobilized Ni2+ ions. The chelating group of the Ni-IDA resin is based on iminodiacetic acid (IDA), which enables strong and efficient binding of target protein to the IMAC matrix. In contrast to traditional IDA matrices, MoBiTec Ni-IDA matrix is an optimized matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the adsorbent. As a result, Lab Columns Ni-IDA and Mobicol Spin Ni-IDA columns provide much higher target protein purity. Mobicol Spin Ni-IDA Columns: • Pre-packed, single-use spin columns for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. • For protein purification by spinning • Compatible with standard microcentrifuges • The purification run takes approximately 10 min • Very high binding capacity: up to 12 mg protein per spin column • Excellent protein recovery rate of > 90% • No commercial license required |
| Order #: | PR-HTK105 |
| Unit Size: | 5 columns |
| Supplier: | MoBiTec GmbH |
| Shipping: | RT |
| Storage: | RT |
| Subcategory: | Protein Purification |
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