Fluorescein labeled K63-Ub2 (UBPBio Product Code: D2110)
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| Description: | This product contains an authentic K63-linked isopeptide bond, synthesized by using an enzymatic reaction containing UbE1, UbE2N, Ub(K63R) and Ub-fluorescein. Fluorescein is labeled on the C-terminus of Ub by mutating glycine 76 to a cysteine. In fluorescein labeled K63 Ub2, fluorescein is labeled on the proximal Ub. It is an excellent tool for monitoring deubiquitination that can be visualized by in-gel fluorescent detection of the production of fluorescein labeled monoUb (excitation at ~490 nm, and see product data sheet for examples). The assay is more quantitative and sensitive than immunoblotting of Ub. The detection limit is 10 ng or less. |
| Order #: | UBP-D2110-25 |
| Unit Size: | 25 µg |
| Supplier: | UBPBio |
| Restrictions: | Only available in selected countries. |
| Shipping: | Dry ice |
| Storage: | -80 °C |
| Subcategory: | Ubiquitin / Sumo Chains |
| More information: | Go to webpage |
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