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E. coli p3T vector for PCR Product Cloning

E. coli p3T vector for PCR Product Cloning
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Description: PCR Cloning Vector p3T. The p3T vector provides a flexible system for the direct cloning of PCR products. It allows cloning of PCR products via multiple dT overhangs. Due to a unique series of restriction sites the p3T vector can be cleaved by different enzymes (XcmI, BcgI or PflMI) to produce 1, 2 or 3 T (thymin) overhangs. These permits either the direct cloning of PCR products via a single A (adenin) extension or polyadenylating the PCR fragment and cloning via multiple A extensions. If the PCR fragment is polyadenylated using terminal deoxynucleotidyl transferase, it can be cloned with high efficiency. The polyadenylation is a simple procedure requiring only a five-minute reaction time. Features: • Multiple Cloning Site with diverse cloning options: 1. Direct cloning of PCR products (single dA extension) 2. Cloning of polyadenylated fragments • Enables efficient ligation and requires low amounts of insert DNA • Linearization site (SmaI) to reduce background of “empty” vector clones • BalI sites flank MCS for optimal excision of the insert • High-efficiency-cloning • Blue/white selection by α-complementation
Order #: P123T
Unit Size: 5 µg
Supplier: MoBiTec GmbH
Shipping: RT
Storage: 4 °C
Subcategory: E. coli Expression Systems
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