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pBBR RESO Broad Host Range vector for promotor identification

pBBR RESO Broad Host Range vector for promotor identification
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Description: Promoter-Trap Vector pBBR RESO (Broad-Host-Range) - Identify promoters in a wide variety of gram-negative bacteria. The plasmid pBBR RESO is a broad-host-range promoter cloning vector. In contrast to other known broad-host-range vectors, it is maintained at a medium copy number and has a reasonable size of about 6.8 kb. It stably replicates in any gram-negative bacterium studied, and is therefore particularly interesting for the isolation and genetic analysis of DNA sequences with promoter activity in the homologous organism. The reporter system used employs the resolvase-mediated excision of a kanamycin (Kan)-resistance gene flanked by two res sequences. Cloning an active promoter results in Kan-sensitive clones. For identification of: promoter sequence of a cloned gene or operon • promoter sequences in random tnpR-gene fusion libraries • genes induced under harsh environmental conditions, such as various stresses, where other reporter genes and selection systems (cat, bla) cannot be used
Order #: RESO01
Unit Size: 5 µg
Supplier: MoBiTec GmbH
Shipping: RT
Storage: 4 °C
Subcategory: E. coli Expression SystemsE. coli Expression SystemsE. coli Expression SystemsE. coli Expression Systems
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