mobitec-globe Innovative Tools for Molecular and Cell Biology

Live-Cell Assays for COVID-19 Drug Screening

Fluorescent Assays for SARS-CoV-2 Viral Entry

  • Detect host-pathogen interaction in living cells
  • Identify therapeutic drugs that block ACE2-mediated viral entry
  • Screen for neutralizing antibodies to SARS-CoV-2 spike and emerging spike variants
  • Cost effective screening with minimal safety requirements
  • Your choice of host cell

SARS-CoV-2 Pseudovirus

This pseudovirus is a modified BacMam vector that presents SARS-CoV-2 spike protein on the surface of the BacMam capsid. The spike protein facilitates entry into cells expressing ACE2 and TMPRSS2. Once inside the cell, BacMam does not replicate, but delivers a genetically encoded, fluorescent reporter that expresses bright red or green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host cell nucleus is dark. Pseudo SARS-CoV-2 is safe to use with no risk of infection and requires only BSL-1 handling.

Pseudo Host Cells

To transform any cell into pseudo host cells, we offer red and green fluorescent ACE2 and TMPRSS2 in BacMam expression vectors. After transduction host cells are ready for the viral entry assay. If the pseudovirus enters the cells, it will produce bright fluorescence in the nucleus. A significant advantage of creating pseudo host cells is that compounds blocking viral entry can be screened using standard cell lines that are compatible with high-throughput screening.

Read more:

Reagents for SARS-CoV-2 Research - BacMam pseudoviruses and “pseudo host” vectors carrying host-cell proteins for viral entry.

SARS-CoV-2 Entry - Host Cell Factors for SARS-CoV-2 Viral Entry

SARS CoV-2 Pseudoviruses - Tools for Measuring SARS-CoV-2 Entry

For the latest updates about COVID-19 tools for research and drug discovery, visit the NEWS page of Montana Molecular

Pseudovirus Products

Product Details: Pseudo SARS-CoV-2 Red Reporter

Product Details: Pseudo SARS-CoV-2 Green Reporter

Product Details: Pseudo SARS-CoV-2 D614G Mutation Red Reporter

Product Details: Pseudo SARS-CoV-2 D614G Mutation Green Reporter

Host Products

Product Details: Angiotensin Converting Enzyme 2 (ACE2)-Green

Product Details: Angiotensin Converting Enzyme 2 (ACE2)-Red

Product Details: TMPRSS2

Product Details: Neuropilin 1

Product Details: Cathepsin L

*All prices in € excl. VAT and shipping