TFF-Easy: Tangential Flow Filter for EV Concentration
- A unique concentrator suitable for all EV containing samples, e.g., cell media or urine
- Easy removal of small molecules and ions from the EV preparation
- Reusable multiple times
- Facilitates EV dialysis to change buffer composition
Efficient Concentration of Diluted Fluids for EV Purification
Tangential flow filtration (TFF) is a rapid and efficient method, usually used for separation and purification of biomolecules. TFF can also be used to concentrate and desalt sample solutions, and is emerging as a new technique for easily isolating Extracellular Vesicle (EVs), if coupled with SEC.
HansaBioMed’s TFF-Easy is a filter cartridge containing hollow fibers made of polysulfone suitable for the concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification.
TFF-Easy can be used manually with syringes of different size, for concentrating small volumes of fluids, or can be connected to a mechanical pump for concentrating larger volumes.
TFF-MV: Filter for Large EV Separation and Purification
TFF-MV is a filter able to separate large microvesicles (MVs) by size, avoiding the separation by centrifugation at 10000 g, which often causes the loss of part of small EVs. TFF-MV retains vesicles larger than 150-200 nm, whereas small EVs and circulating molecules pass in the permeate. Retained MVs can be recovered with a syringe in PBS buffer, without additional purification steps.
TFF-EVs: Fast EV Purification
TFF-EVs is a next-generation filter which allows a rapid, reproducible and scalable purification of EVs. It can be used on the lab bench for purifying small sample volumes (min 5 ml) or connected with a mechanical system for purifying larger volumes.
TFF-EVs is a filter cartridge made of polyethersulfone hollow fibers with pores of 50 nm size (cut off 300 kDa). The filter allows the purification of EVs and particles > 50 nm.