Huge Selection of Cloning and Expression Vectors
MoBiTec is leading in the development of unique and cutting edge vector systems for recombinant protein expression. We offer a huge selection of easy-to-handle tools for efficient gene expression in different microorganisms like Bacillus megaterium, Bacillus subtilis, Lactococcus lactis, Escherichia coli, and yeast. Expression systems for mammalian cells as well as vector systems for many other purposes – like localization studies of cells, cell compartments, and fusion proteins in live cells - complete our vector portfolio.
In addition to the vectors, several useful host strains are available, including ready-to-use protoplasts for Bacillus megaterium which is difficult to transform.
Bacillus megaterium Expression Systems
Features
- Versatile protein production (intracellular and extracellular)
- Ideal for small and large scale protein production
- Tightly regulated & efficiently inducible xylA promoter
- Also constitutive expression systems available
- No alkaline protease activity and no endotoxins
- High performance vectors
- Vectors with two ribosome binding sites (2RBS) for simultaneous dual expression
- Versatile purification (6xHis-tag, Strep-tag, Strep/6xHis double-tag)
- Vectors with removable purification tag
- Protoplasts specifically optimized for transformation
Please note, a license is required for any non-academic customer. Please contact us for more information.
Availabe Systems and Downloads
- Bacillus megaterium Classic Expression Vectors ( pdf)
- Bacillus megaterium High Performance Expression Vectors ( pdf)
- Bacillus megaterium T7-RNA Polymerase Expression Vectors ( pdf)
- Bacillus megaterium Constitutive Expression System ( pdf)
Bacillus subtilis Expression Systems
Features
- Tightly controlled gene expression in a bacterium with long history in biotechnology
- Intracellular expression or secretion
- Avoid expensive inducers (e.g., IPTG)
- Reduced protease activity
- Prevent protein aggregation
- Controlled gene expression for metabolic engineering
- E. coli / B. subtilis shuttle vectors with different tags (His-tag, Strep-tag)
- Endotoxin-free
- Potentially useful for cloning of genes with toxic product
- Easy transformation
- New vector available for high-level extracellular protein production
Please note, a license is required for any non-academic customer. Please contact us for more information.
Availabe Systems and Downloads
- Bacillus subtilis Epitope- and Fluorescence-Tagging Integration Vectors ( pdf)
- Bacillus subtilis Pgrac01 Expression Vectors ( pdf)
- Bacillus subtilis Pgrac100 Expression Vectors (High-Yield Protein Production) ( pdf)
- Bacillus subtilis SURE® Expression System ( pdf)
- Bacillus subtilis Food Grade Expression System ( pdf)
Lactococcus lactis Expression Systems
Features
- Stable, high-yield protein production; easy-to-handle
- Metabolite engineering
- Intracellular expression or secretion
- Food grade expression system
- Inducible and constitutive expression systems
- Endotoxin-free
- No inclusion bodies
- Reduced protease activity
- Expression of genes with toxic products
- Expression of membrane proteins
- Suitable also for other lactic acid bacteria
Please note, a license is required for any non-academic customer. Please contact us for more information.
Availabe Systems and Downloads
- Constitutive Gene Expression System for Lactococcus lactis and Other Lactic Acid Bacteria ( pdf)
- Lactococcus lactis NICE® Expression System ( pdf)
