mobitec-globe Innovative Tools for Molecular and Cell Biology

Vector Systems

Huge Selection of Cloning and Expression Vectors

MoBiTec is leading in the development of unique and cutting edge vector systems for recombinant protein expression. We offer a huge selection of easy-to-handle tools for efficient gene expression in different microorganisms like Bacillus megaterium, Bacillus subtilis, Lactococcus lactis, Escherichia coli, and yeast. Expression systems for mammalian cells as well as vector systems for many other purposes – like localization studies of cells, cell compartments, and fusion proteins in live cells - complete our vector portfolio.

In addition to the vectors, several useful host strains are available, including ready-to-use protoplasts for Bacillus megaterium which is difficult to transform.

Bacillus megaterium Expression Systems

Features

  • Versatile protein production (intracellular and extracellular)
  • Ideal for small and large scale protein production
  • Tightly regulated & efficiently inducible xylA promoter
  • Also constitutive expression systems available
  • No alkaline protease activity and no endotoxins
  • High performance vectors
  • Vectors with two ribosome binding sites (2RBS) for simultaneous dual expression
  • Versatile purification (6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Vectors with removable purification tag
  • Protoplasts specifically optimized for transformation
Bacillus megaterium Expression Systems

Please note, a license is required for any non-academic customer. Please contact us for more information.

  Availabe Systems and Downloads

  • Bacillus megaterium Classic Expression Vectors ( pdf)
  • Bacillus megaterium High Performance Expression Vectors ( pdf)
  • Bacillus megaterium T7-RNA Polymerase Expression Vectors ( pdf)
  • Bacillus megaterium Constitutive Expression System ( pdf)

Bacillus subtilis Expression Systems

Features

  • Tightly controlled gene expression in a bacterium with long history in biotechnology
  • Intracellular expression or secretion
  • Avoid expensive inducers (e.g., IPTG)
  • Reduced protease activity
  • Prevent protein aggregation
  • Controlled gene expression for metabolic engineering
  • E. coli / B. subtilis shuttle vectors with different tags (His-tag, Strep-tag)
  • Endotoxin-free
  • Potentially useful for cloning of genes with toxic product
  • Easy transformation
  • New vector available for high-level extracellular protein production
Bacillus megaterium Expression Systems

Please note, a license is required for any non-academic customer. Please contact us for more information.

  Availabe Systems and Downloads

  • Bacillus subtilis Epitope- and Fluorescence-Tagging Integration Vectors ( pdf)
  • Bacillus subtilis Pgrac01 Expression Vectors ( pdf)
  • Bacillus subtilis Pgrac100 Expression Vectors (High-Yield Protein Production) ( pdf)
  • Bacillus subtilis SURE® Expression System ( pdf)
  • Bacillus subtilis Food Grade Expression System ( pdf)

Lactococcus lactis Expression Systems

Features

  • Stable, high-yield protein production; easy-to-handle
  • Metabolite engineering
  • Intracellular expression or secretion
  • Food grade expression system
  • Inducible and constitutive expression systems
  • Endotoxin-free
  • No inclusion bodies
  • Reduced protease activity
  • Expression of genes with toxic products
  • Expression of membrane proteins
  • Suitable also for other lactic acid bacteria
Bacillus megaterium Expression Systems

Please note, a license is required for any non-academic customer. Please contact us for more information.

  Availabe Systems and Downloads

  • Constitutive Gene Expression System for Lactococcus lactis and Other Lactic Acid Bacteria ( pdf)
  • Lactococcus lactis NICE® Expression System ( pdf)
Huge Selection of Cloning and Expression Vectors MoBiTec is leading in the development of unique and cutting edge vector systems for recombinant protein expression. We offer a huge... read more »
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Vector Systems

Huge Selection of Cloning and Expression Vectors

MoBiTec is leading in the development of unique and cutting edge vector systems for recombinant protein expression. We offer a huge selection of easy-to-handle tools for efficient gene expression in different microorganisms like Bacillus megaterium, Bacillus subtilis, Lactococcus lactis, Escherichia coli, and yeast. Expression systems for mammalian cells as well as vector systems for many other purposes – like localization studies of cells, cell compartments, and fusion proteins in live cells - complete our vector portfolio.

In addition to the vectors, several useful host strains are available, including ready-to-use protoplasts for Bacillus megaterium which is difficult to transform.

Bacillus megaterium Expression Systems

Features

  • Versatile protein production (intracellular and extracellular)
  • Ideal for small and large scale protein production
  • Tightly regulated & efficiently inducible xylA promoter
  • Also constitutive expression systems available
  • No alkaline protease activity and no endotoxins
  • High performance vectors
  • Vectors with two ribosome binding sites (2RBS) for simultaneous dual expression
  • Versatile purification (6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Vectors with removable purification tag
  • Protoplasts specifically optimized for transformation
Bacillus megaterium Expression Systems

Please note, a license is required for any non-academic customer. Please contact us for more information.

  Availabe Systems and Downloads

  • Bacillus megaterium Classic Expression Vectors ( pdf)
  • Bacillus megaterium High Performance Expression Vectors ( pdf)
  • Bacillus megaterium T7-RNA Polymerase Expression Vectors ( pdf)
  • Bacillus megaterium Constitutive Expression System ( pdf)

Bacillus subtilis Expression Systems

Features

  • Tightly controlled gene expression in a bacterium with long history in biotechnology
  • Intracellular expression or secretion
  • Avoid expensive inducers (e.g., IPTG)
  • Reduced protease activity
  • Prevent protein aggregation
  • Controlled gene expression for metabolic engineering
  • E. coli / B. subtilis shuttle vectors with different tags (His-tag, Strep-tag)
  • Endotoxin-free
  • Potentially useful for cloning of genes with toxic product
  • Easy transformation
  • New vector available for high-level extracellular protein production
Bacillus megaterium Expression Systems

Please note, a license is required for any non-academic customer. Please contact us for more information.

  Availabe Systems and Downloads

  • Bacillus subtilis Epitope- and Fluorescence-Tagging Integration Vectors ( pdf)
  • Bacillus subtilis Pgrac01 Expression Vectors ( pdf)
  • Bacillus subtilis Pgrac100 Expression Vectors (High-Yield Protein Production) ( pdf)
  • Bacillus subtilis SURE® Expression System ( pdf)
  • Bacillus subtilis Food Grade Expression System ( pdf)

Lactococcus lactis Expression Systems

Features

  • Stable, high-yield protein production; easy-to-handle
  • Metabolite engineering
  • Intracellular expression or secretion
  • Food grade expression system
  • Inducible and constitutive expression systems
  • Endotoxin-free
  • No inclusion bodies
  • Reduced protease activity
  • Expression of genes with toxic products
  • Expression of membrane proteins
  • Suitable also for other lactic acid bacteria
Bacillus megaterium Expression Systems

Please note, a license is required for any non-academic customer. Please contact us for more information.

  Availabe Systems and Downloads

  • Constitutive Gene Expression System for Lactococcus lactis and Other Lactic Acid Bacteria ( pdf)
  • Lactococcus lactis NICE® Expression System ( pdf)

Please Browse our Vector Systems Products:

 
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pNZ7025 Lactococcus lactis const. vector, pepN, food grade, (alr)
Unit: 10 µg
Price on request
Datasheet
E. coli host strain MC1061
E. coli host strain MC1061 Order #: VS-ELS10610-01
Unit: 1 ml
Price on request
Datasheet
E. coli host strain MC1061, Chemically Competent
Unit: 5 x 100 µl
Price on request
Datasheet
NICE® pNZ8008 Reference plasmid with gusA gene
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ8148 Lactococcus lactis expression vector, NcoI site
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ8150 Lactococcus lactis expression vector, ScaI site
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ8149 Lactococcus lactis expression vector, food grade (lacF), NcoI site
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ8151 Lactococcus lactis expression vector, food grade (lacF), Scal site
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ8152 Lactococcus lactis expression vector, food grade (alr), Scal site
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000
Unit: 1 ml
Price on request
Datasheet
NICE® pNZ8120 Lactococcus lactis secretion vector (SP PrtP/NaeI)
Unit: 10 µg
Price on request
Datasheet
NICE® pNZ8121 Lactococcus lactis secretion vector (SP PrtP/EcoRV)
Unit: 10 µg
Price on request
Datasheet
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